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mitochondria isolation kit  (Miltenyi Biotec)


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    Miltenyi Biotec mitochondria isolation kit
    Mitochondria Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitochondria isolation kit/product/Miltenyi Biotec
    Average 95 stars, based on 125 article reviews
    mitochondria isolation kit - by Bioz Stars, 2026-02
    95/100 stars

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    ( A ) Differential analysis of mitochondrial proteomics in A2780 and SKOV3 cells, and enrichment of downregulated biological processes in SKOV3 cells compared with A2780 cells. p values are all presented in scientific notation (4e-06 means 4 × 10 −6 ); ( B ) Upregulated small ribosomal subunits in A2780 cells compared with SKOV3 cells; ( C ) Upregulated large ribosomal subunits in A2780 cells compared with SKOV3 cells; ( D ) Enrichment of proteins related to complex IV assembly, translation, and copper ion transport that are upregulated in A2780 cells compared with SKOV3 cells; ( E ) Western Blot detection of the <t>mitochondria</t> protein expression changes. The area above the dotted line represents the fold increase in gene expression of A2780 compared to SKOV3 cells in ( B – D ).
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    ( A ) Differential analysis of mitochondrial proteomics in A2780 and SKOV3 cells, and enrichment of downregulated biological processes in SKOV3 cells compared with A2780 cells. p values are all presented in scientific notation (4e-06 means 4 × 10 −6 ); ( B ) Upregulated small ribosomal subunits in A2780 cells compared with SKOV3 cells; ( C ) Upregulated large ribosomal subunits in A2780 cells compared with SKOV3 cells; ( D ) Enrichment of proteins related to complex IV assembly, translation, and copper ion transport that are upregulated in A2780 cells compared with SKOV3 cells; ( E ) Western Blot detection of the mitochondria protein expression changes. The area above the dotted line represents the fold increase in gene expression of A2780 compared to SKOV3 cells in ( B – D ).

    Journal: International Journal of Molecular Sciences

    Article Title: LRPPRC-Driven Oxidative Phosphorylation Is Associated with Elesclomol-Induced Cuproptosis in Ovarian Cancer

    doi: 10.3390/ijms27010451

    Figure Lengend Snippet: ( A ) Differential analysis of mitochondrial proteomics in A2780 and SKOV3 cells, and enrichment of downregulated biological processes in SKOV3 cells compared with A2780 cells. p values are all presented in scientific notation (4e-06 means 4 × 10 −6 ); ( B ) Upregulated small ribosomal subunits in A2780 cells compared with SKOV3 cells; ( C ) Upregulated large ribosomal subunits in A2780 cells compared with SKOV3 cells; ( D ) Enrichment of proteins related to complex IV assembly, translation, and copper ion transport that are upregulated in A2780 cells compared with SKOV3 cells; ( E ) Western Blot detection of the mitochondria protein expression changes. The area above the dotted line represents the fold increase in gene expression of A2780 compared to SKOV3 cells in ( B – D ).

    Article Snippet: Mitochondria extraction was conducted according to the instructions of the Mitochondria Isolation kit (Invent Biotechnologies, Eden Prairie, MN, USA).

    Techniques: Western Blot, Expressing, Gene Expression

    ( A ) Analysis of the difference in FDX1 protein expression between normal and ovarian cancer tissues using the UALCAN website; ( B ) Analysis of the PPI between FDX1 and complex IV using the STRING database; ( C ) The levels of proteins related to cuproptosis extracted from isolated mitochondria upregulate in A2780 cells compared with SKOV3 cells. The area above the dotted line represents the fold increase in gene expression of A2780 compared to SKOV3 cells; ( D ) Western Blot detection of the whole-cell expression changes in the death-related protein LRPPRC, MTCO1, FDX1, lipoic acid synthesis proteins LIPT1 and LIAS, and copper ion transport proteins ATP7B and CTR1 (SLC31A1) in A2780 and SKOV3 cells after treatment with Elesclomol + CuCl 2 (1:1). ( E , F ) After reatment with 0.1 μM Elesclomol + CuCl 2 (1:1), the activity of complex IV were detected in A2780 and SKOV3 cells. * p < 0.05, *** p < 0.001 compared with the CON(Control) group.

    Journal: International Journal of Molecular Sciences

    Article Title: LRPPRC-Driven Oxidative Phosphorylation Is Associated with Elesclomol-Induced Cuproptosis in Ovarian Cancer

    doi: 10.3390/ijms27010451

    Figure Lengend Snippet: ( A ) Analysis of the difference in FDX1 protein expression between normal and ovarian cancer tissues using the UALCAN website; ( B ) Analysis of the PPI between FDX1 and complex IV using the STRING database; ( C ) The levels of proteins related to cuproptosis extracted from isolated mitochondria upregulate in A2780 cells compared with SKOV3 cells. The area above the dotted line represents the fold increase in gene expression of A2780 compared to SKOV3 cells; ( D ) Western Blot detection of the whole-cell expression changes in the death-related protein LRPPRC, MTCO1, FDX1, lipoic acid synthesis proteins LIPT1 and LIAS, and copper ion transport proteins ATP7B and CTR1 (SLC31A1) in A2780 and SKOV3 cells after treatment with Elesclomol + CuCl 2 (1:1). ( E , F ) After reatment with 0.1 μM Elesclomol + CuCl 2 (1:1), the activity of complex IV were detected in A2780 and SKOV3 cells. * p < 0.05, *** p < 0.001 compared with the CON(Control) group.

    Article Snippet: Mitochondria extraction was conducted according to the instructions of the Mitochondria Isolation kit (Invent Biotechnologies, Eden Prairie, MN, USA).

    Techniques: Expressing, Isolation, Gene Expression, Western Blot, Activity Assay, Control